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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which results in chromosome instability, and thus may well contribute to tumorigenesis. This protein was observed to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important role in promoting mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two added splice variants ended up described but specific nucleotide sequence info just isn't however out there.
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Blocking peptides are peptides that bind especially towards the focus on antibody and block antibody binding. These peptide typically incorporates the epitope acknowledged via the antibody.
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This guide outlines further more possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This information outlines even more doable optimization of mobile and immunoassay parameters to be sure the very best effects are...
Antibodies sure to the blocking peptide now not bind into the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is specific.
Target Information Cyclin E belongs into the hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute on the temporal coordination of each mitotic event. Cyclin E kinds a posh with and features being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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